ABSTRACT
Our aim was to investigate the role of chemokines in promoting instability of coronary atherosclerotic plaques and the underlying molecular mechanism. Coronary angiography and intravascular ultrasound (IVUS) were performed in 60 stable angina pectoris (SAP) patients and 60 unstable angina pectoris (UAP) patients. The chemotactic activity of monocytes in the 2 groups of patients was examined in Transwell chambers. High-sensitivity C-reactive protein (hs-CRP), monocyte chemoattractant protein-1 (MCP-1), regulated on activation in normal T-cell expressed and secreted (RANTES), and fractalkine in serum were examined with ELISA kits, and expression of MCP-1, RANTES, and fractalkine mRNA was examined with real-time PCR. In the SAP group, 92 plaques were detected with IVUS. In the UAP group, 96 plaques were detected with IVUS. The plaques in the UAP group were mainly lipid 51.04% (49/96) and the plaques in the SAP group were mainly fibrous 52.17% (48/92). Compared with the SAP group, the plaque burden and vascular remodeling index in the UAP group were significantly greater than in the SAP group (P<0.01). Chemotactic activity and the number of mobile monocytes in the UAP group were significantly greater than in the SAP group (P<0.01). Concentrations of hs-CRP, MCP-1, RANTES, and fractalkine in the serum of the UAP group were significantly higher than in the serum of the SAP group (P<0.05 or P<0.01), and expression of MCP-1, RANTES, and fractalkine mRNA was significantly higher than in the SAP group (P<0.05). MCP-1, RANTES, and fractalkine probably promote instability of coronary atherosclerotic plaque.
Subject(s)
Adult , Female , Humans , Male , Middle Aged , Angina Pectoris/metabolism , Chemokines/metabolism , Chemotaxis/physiology , Coronary Artery Disease/metabolism , Monocytes/metabolism , Plaque, Atherosclerotic/physiopathology , Angina Pectoris/physiopathology , C-Reactive Protein/analysis , /blood , /blood , /blood , Coronary Artery Disease/physiopathology , Real-Time Polymerase Chain Reaction , Ultrasonography, InterventionalABSTRACT
The LHX3 gene encodes LIM homeodomain class transcription factors that have important roles to play in pituitary and nervous system development.On the one hand,mutations of LHX3 are associated with deficiencies of growth hormone (GH),prolactin (PRL),luteotrophic hormone (LH),follicle-stimulating hormone (FSH)and thyroid- stimulating hormone (TSH);on the other hand,mutations of LHX3 are also associated with combined pituitary hormone deficiency (CPHD) diseases in human and animal models.To date,few polymorphisms of the bovine LHX3 gene have been reported.In this study,polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP)and DNA sequencing methods were employed to screen the genetic variations within the bovine LHX3 gene in 802 Chinese indigenous cattle.The results revealed three novel single-nucleotide polymorphisms (SNPs): AY923832: g.7553G >A, 7631C>T and 7668C>G.Among them,a synonymous mutation of exon II was identified: GAG ((Glu)> GAA (Glu) at position 72 aa (AY923832:g.7553G>A) of LHX3 ((403aa) in the four Chinese bovine breeds.Significant statistical differences in genotypic frequencies for exon II and its flanking region of the LHX3 gene implied that the polymorphic locus was significantly associated with cattle breeds by the X2-test (X2=68.975,df=6, P <0.001).Hence,the three novel SNPs not only extend the spectrum of genetic variations of the bovine LHX3 gene, but could also possibly contribute to conducting association analysis and evaluating these as genetic markers in bovine breeding and genetics,and CPHD detection.